DESCRIPTION

The SK7 (Leu-4) monoclonal antibody specifically binds to the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa). The CD3 antigen is present on 61% to 85% of normal peripheral blood lymphocytes 60% to 85% of thymocytes and on Purkinje cells in cerebellum. The soluble form of this antibody has a mitogenic effect on most peripheral blood T lymphocytes, provided appropriate functional monocytes are present.

BD CD3抗體（PE-CY7標記）557851

Brand

BD Pharmingen™

Alternative Name

CD3-epsilon; CD3E; Leu4; T-cell surface antigen T3/Leu-4 epsilon chain; T3E

Vol. Per Test

5 µl

Isotype

Mouse BALB/c IgG1, κ

Reactivity

Human (QC Testing)

Application

Flow cytometry (Routinely Tested)

Immunogen

Human Thymocytes

Workshop No.

II T118; III T492

Storage Buffer

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

557851

FORMAT

Format

PE-Cy™7

Excitation Source

Blue 488 nm,Green 532 nm,Yellow/Green 561 nm

Excitation Max

496 nm

Emission Max

785 nm

PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.